Assessment of immunoglobulin M enzyme immunoassays for diagnosis of measles.

نویسندگان

  • Graham A Tipples
  • Rasool Hamkar
  • Talat Mohktari-Azad
  • Michael Gray
  • Geoff Parkyn
  • Carol Head
  • Samuel Ratnam
چکیده

We evaluated the performance of three commercial measles immunoglobulin M enzyme immunoassays from Meddens, Denka Seiken, and Behring. The sensitivities were determined to be 96.7% for the Meddens and Denka Seiken assays and 87.9% for the Behring assay. The specificities of the assays were determined to be 94.6% for Meddens, 98.2% for Denka Seiken, and 98.7% for Behring.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Optimal Timing of Specimen Collection after Rash Onset for Diagnosis of Measles IgM Antibody

  Antibody detection is the most versatile and commonly used method for measles diagnosis. Detection of specific IgM antibodies in a single serum specimen collected within the appropriate time after rash onset can provide a good presumptive diagnosis of current or recent measles infection and is the test of choice for rapid diagnosis of measles cases. So, optimal timing for collection of a sing...

متن کامل

Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies.

We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4 degrees C until assayed. Each assay m...

متن کامل

Production of a monoclonal antibody against chicken immunoglobulin G: A valuable molecule with research and diagnostic applications

Monoclonal antibodies (MAbs) are invaluable molecules which have several advantages over polyclonal immunoglobulins (Igs) including consistency and higher specificity and hence can be used in biological researches, diagnosis and treatment of diseases. The present study was conducted to produce monoclonal antibody against chicken IgG.TheIgG molecules were purified from chicken serum and used as ...

متن کامل

Evaluation of Immune Status to Measles in Vaccinated Population in Tehran, by Enzyme-linked Immunosorbent Assay and the Hemagglutination Inhibition Techniques (1386-1387)

Background and Aims: Measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. Although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including Iran. Methods: The present study was performed to evaluate the immune status aga...

متن کامل

Evaluation of immune status to measles in vaccinated population in Tehran, using enzyme-linked immunosorbent assay and the hemagglutination inhibition techniques

Abstract: Measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. Although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including Iran. Understanding measles outbreaks that occur after the initiation of measles eliminati...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 41 10  شماره 

صفحات  -

تاریخ انتشار 2003